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Z02 - Quantitative Proteomics

Abstract

The quantitative proteome analysis is a key technology for most of the projects within the CRC 1218. The aim of the Z02 project is to support the projects with mass spectrometric expertise. Besides the assessment of global protein expression profiles, the major focus of the facility is the identification of protein-protein interaction in order to decipher molecular networks within mitochondria. In addition, we will investigate activated signalling pathways by the enrichment of post translational modifications, including phosphorylated and ubiquitinated peptides. Moreover, the Z02 project will also support the CRC with statistical and bioinformatics data analysis.

Project-related publications

Nolte, H., MacVicar, T.D., Tellkamp, F., Krüger, M. (2018). Instant Clue: A Software Suite for Interactive Data Visualization and Analysis. Sci Rep. 8, 12648.

Wiederstein, J.L., Nolte, H., Günther, S., Piller, T., Baraldo, M., Kostin, S., Bloch, W., Schindler, N., Sandri, M., Blaauw, B., Braun, T., Hölper, S., Krüger, M. (2018). Skeletal Muscle-Specific Methyltransferase METTL21C Trimethylates p97 and Regulates Autophagy-Associated Protein Breakdown. Cell Rep. 23, 1342-1356.

Saita, S., Nolte, H., Fiedler, K.U., Kashkar, H., Venne, A.S., Zahedi, R.P., Krüger, M., and Langer, T. (2017). PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis. Nat. Cell Biol. 19, 318-328.

Islam, M.S., Nolte, H., Jacob, W., Ziegler, A.B., Pütz, S., Grosjean, Y., Szczepanowska, K., Trifunovic, A., Braun, T., Heumann, H., Heumann, R., Hovemann, B., Moore, D.J., Krüger, M. (2016). Human R1441C LRRK2 regulates the synaptic vesicle proteome and phosphoproteome in a Drosophila model of Parkinson's disease. Hum. Mol. Genet. 25, 5365-5382.

Al-Furoukh, N., Ianni, A., Nolte, H., Holper, S., Krüger, M., Wanrooij, S., and Braun, T. (2015). ClpX stimulates the mitochondrial unfolded protein response (UPRmt) in mammalian cells. Biochim. Biophys. Acta 1853, 2580-2591.