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B02 - Hydrogen peroxide signalling in mitochondrial communication

Jan Riemer
Institute for Biochemistry
University of Cologne

E-mail: jan.riemer(at)uni-koeln.de
Phone: +49 - 221 / 470 7306
For more information and contact please visit the RIEMER LAB.

Abstract
The release of hydrogen peroxide (H2O2) from mitochondria has been implicated in regulating processes inside and outside of the organelle. H2O2 is an elegant choice as mitochondrial signalling molecule because its production integrates inputs from mitochondrial activity, the availability of redox enzymes and cellular metabolism. At present it is unclear how much H2O2 is produced by mitochondria in intact cells and what protein targets of H2O2 signalling are. We will investigate the spatiotemporal pattern of mitochondrial H2O2 release using novel genetically-encoded fluorescent sensors. We will identify targets of mitochondria-derived H2O2 using quantitative redox proteomics. We will thereby answer fundamental questions on the where, when and how of mitochondrial H2O2 production, and on the role of H2O2 in coordinating mitochondrial function with the rest of the cell.

Latest publications

Friederich, M.W., Erdogan, A.J., Coughlin, C.R., Elos, M.T., Jiang, H., O'Rourke, C.P., Lovell, M.A., Wartchow, E., Gowan, K., Chatfield, K.C., Chick, W.S., Spector, E.B., Van Hove, J.L., and Riemer J. (2016). Mutations in the accessory subunit NDUFB10 result in isolated complex I deficiency and illustrate the critical role of intermembrane space import for complex I holoenzyme assembly. Hum Mol Genet. pii: ddw431. doi: 10.1093/hmg/ddw431. [Epub ahead of print]

Petrungaro, C., Zimmermann, K.M., Küttner, V., Fischer, M., Dengjel, J., Bogeski, I., and Riemer, J. (2015). The Ca2+-dependent release of the Mia40-induced MICU1-MICU2 dimer from MCU regulates mitochondrial Ca2+ uptake. Cell Metab. 22, 721-733.