Z02 | BIOANALYTICAL PLATFORM
Christian Frezza
CECAD Research Center
University Hospital Cologne
E-mail: christian.frezza@uni-koeln.de
Phone: +49 (0) 221 478 84308
For more information and contact please visit the FREZZA LAB.
Marcus Krüger
CECAD Research Center/Institute for Genetics
University of Cologne
E-mail: marcus.krueger
uni-koeln.de
Phone: +49 - 221 / 478 84140
For more information and contact please visit Proteomics Facility & KRÜGER LAB.
Aleksandra Trifunovic
CECAD Research Center/Institute for Mitochondrial Diseases and Ageing
University Hospital Cologne
E-mail: aleksandra.trifunovicuk-koeln.de
Phone: +49 - 221 / 478 84291
For more information and contact please visit the TRIFUNOVIC LAB.
Abstract
The Bioanalytical Platform offers an advanced research platform for the projects of CRC 1218 ranging from proteomics and metabolomics to bioenergetics. While proteomics is aimed to identify proteins and their functional networks, metabolomics provides an analytical tool for the identification of pathway alterations and biosignature analyses. The integrative bioanalytical platform is an integral part of the CRC 1218, and the facilities have established a diverse setup of mass spectrometry-based proteomics and metabolomics methods, as well as various bioenergetic approaches that enables the analysis of dynamics of mitochondria in living organisms.
Project-related publications
Moschandrea C, Kondylis V, Evangelakos I, Herholz M, Schneide F, Schmidt C, Yang M, Ehret S, Heine H, Jaeckstein MJ, Szczepanowska K, Schwarzer R, Baumann L, Bock T, Nikitopoulou E, Brodesser S, Krüger M, Frezza C, Heeren J, Trifunovic A, Pasparakis M (2024). Mitochondrial dysfunction abrogates dietary lipid processing in enterocytes. Nature 625(7994):385-392. DOI: 10.1038/s41586-023-06857-0. Epub 2023 Dec 20.
Zecchini V, Paupe V, Herranz-Montoya I, Janssen J, Wortel IMN, Morris JL, Ferguson A, Chowdury SR, Segarra-Mondejar M, Costa ASH, Pereira GC, Tronci L, Young T, Nikitopoulou E, Yang M, Bihary D, Caicci F, Nagashima S, Speed A, Bokea K, Baig Z, Samarajiwa S, Tran M, Mitchell T, Johnson M, Prudent J, Frezza C (2023). Fumarate induces vesicular release of mtDNA to drive innate immunity. Nature 615:499-506. DOI: 10.1038/s41586-023-05770-w.
Hermeling JC, Herholz M, Baumann L, Cores EC, Zecic A, Hoppe T, Riemer J, Trifunovic A (2022). Mitochondria-originated redox signalling regulates KLF-1 to promote longevity in Caenorhabditis elegans. Redox Biol 58:102533. DOI: 10.1016/j.redox.2022.102533.
Sciacovelli M, Dugourd A, Jimenez LV, Yang M, Nikitopoulou E, Costa ASH, Tronci L, Caraffini V, Rodrigues P, Schmidt C, Ryan DG, Young T, Zecchini VR, Rossi SH, Massie C, Lohoff C, Masid M, Hatzimanikatis V, Kuppe C, Von Kriegsheim A, Kramann R, Gnanapragasam V, Warren AY, Stewart GD, Erez A, Vanharanta S, Saez-Rodriguez J, Frezza C (2022). Dynamic partitioning of branched-chain amino acids-derived nitrogen supports renal cancer progression. Nat Commun 13:7830. DOI: 10.1038/s41467-022-35036-4.
Aravamudhan S, Turk C, Bock T, Keufgens L, Nolte H, Lang F, Krishnan RK, Konig T, Hammerschmidt P, Schindler N, Brodesser S, Rozsivalova DH, Rugarli E, Trifunovic A, Bruning J, Langer T, Braun T, Kruger M (2021). Phosphoproteomics of the developing heart identifies PERM1 - An outer mitochondrial membrane protein. J Mol Cell Cardiol 154:41-59. DOI: 10.1016/j.yjmcc.2021.01.010.
Bock T, Turk C, Aravamudhan S, Keufgens L, Bloch W, Rozsivalova DH, Romanello V, Nogara L, Blaauw B, Trifunovic A, Braun T, Kruger M (2021). PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B. Nat Commun 12:4900. DOI: 10.1038/s41467-021-25185-3.
Dugourd A, Kuppe C, Sciacovelli M, Gjerga E, Gabor A, Emdal KB, Vieira V, Bekker-Jensen DB, Kranz J, Bindels EMJ, Costa ASH, Sousa A, Beltrao P, Rocha M, Olsen JV, Frezza C, Kramann R, Saez-Rodriguez J (2021). Causal integration of multi-omics data with prior knowledge to generate mechanistic hypotheses. Mol Syst Biol 17:e9730. DOI: 10.15252/msb.20209730.
Kaspar S, Oertlin C, Szczepanowska K, Kukat A, Senft K, Lucas C, Brodesser S, Hatzoglou M, Larsson O, Topisirovic I, Trifunovic A (2021). Adaptation to mitochondrial stress requires CHOP-directed tuning of ISR. Sci Adv 7. DOI: 10.1126/sciadv.abf0971.
Ryan DG, Yang M, Prag HA, Blanco GR, Nikitopoulou E, Segarra-Mondejar M, Powell CA, Young T, Burger N, Miljkovic JL, Minczuk M, Murphy MP, von Kriegsheim A, Frezza C (2021). Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolism. Elife 10. DOI: 10.7554/eLife.72593.
Willenborg S, Sanin DE, Jais A, Ding X, Ulas T, Nuchel J, Popovic M, MacVicar T, Langer T, Schultze JL, Gerbaulet A, Roers A, Pearce EJ, Bruning JC, Trifunovic A, Eming SA (2021). Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing. Cell Metab 33:2398-2414 e2399. DOI: 10.1016/j.cmet.2021.10.004.