UNIVERSITÄT ZU KÖLN

Susanne Brodesser
Lipidomics/Metabolomics Facility, CECAD Research Center
University Hospital Cologne

E-mail: susanne.brodesseruk-koeln.de
Phone: +49 - 221 / 478 84015
For more information and contact please visit the  Lipidomics/Metabolomics Facility

Marcus Krüger
CECAD Research Center/Institute for Genetics
University of Cologne

E-mail: marcus.kruegeruni-koeln.de
Phone: +49 - 221 / 478 84140
For more information and contact please visit Proteomics Facility & KRÜGER LAB.

Aleksandra Trifunovic
CECAD Research Center/Institute for Mitochondrial Diseases and Ageing
University Hospital Cologne

E-mail: aleksandra.trifunovicuk-koeln.de
Phone: +49 - 221 / 478 84291
For more information and contact please visit the TRIFUNOVIC LAB.

Abstract

The bioanalytical Z project has been developed to meet the challenging demand of a systematic analysis of how mitochondria interfere with cellular functions. The previous Proteomics (Z02) and Bioenergetics (Z04) project are merged with the metabolomics/lipidomics facility to form a central project that provides a broad spectrum of methods to dissect cellular and tissue metabolism at the bioenergetics, metabolite, lipid and proteome level. The new bioanalytical platform (Z02) will be equipped with state-of-the-art mass spectrometers for proteomics and small metabolite analysis. Seahorse and Oxygraph analyzers will provide highest performance for application-oriented basic and advanced bioenergetics investigations of living cells.

Project-related publications

Croon, M., Szczepanowska, K., Popovic, M., Lienkamp, C., Senft, K., Brandscheid, C.P., Bock, T., Gnatzy-Feik, L., Ashurov, A., Acton, R.J., Kaul, H., Pujol, C., Rosenkranz, S., Krüger, M., Trifunovic, A. (2022). FGF21 modulates mitochondrial stress response in cardiomyocytes only under mild mitochondrial dysfunction. Sci Adv. 2022 Apr 8;8(14):eabn7105

Bock, T., Türk, C., Aravamudhan, S., Keufgens, L., Bloch, W., Rozsivalova, D.H., Romanello, V., Nogara, L., Blaauw, B., Trifunovic, A., Braun, T., Krüger, M. (2021). PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B. Nat Commun. 2021 Aug 12;12(1):4900

Kaspar S., Oertlin C., Szczepanowska K., Kukat A., Senft K., Lucas C., Brodesser S., Hatzoglou M., Larsson O., Topisirovic I., Trifunovic A. (2021).
Adaptation to mitochondrial stress requires CHOP-directed tuning of ISR
Science Advances  26 May 2021: Vol. 7, no. 22, eabf0971; DOI: 10.1126/sciadv.abf0971

Aravamudhan, S., Türk, C., Bock, T., Keufgens, L., Nolte, H., Lang, F., Krishnan, R.K., König, T., Hammerschmidt, P., Schindler, N., Brodesser, S., Rozsivalova, D.H., Rugarli, E., Trifunovic, A., Brüning, J., Langer, T., Braun, T., Krüger, M. (2021). Phosphoproteomics of the developing heart identifies PERM1 - An outer mitochondrial membrane protein.  2021 Feb 5;154:41-59. doi.org/10.1016/j.yjmcc.2021.01.010

Schiffmann, L.M., Werthenbach, J. P., Heintges-Kleinhofer, F., Seeger, J.M., Fritsch, M., Günther, S. D., Willenborg, S., Brodesser, S. [1], Lucas, C., Jüngst, C., Albert, M. C., Schorn, F., Witt, A., Moraes, C. T., Bruns, C. J., Pasparakis, M., Krönke, M., Eming, S. A., Coutelle, O., and Kashkar, H. (2020). . Mitochondrial respiration controls neoangiogenesis during wound healing and tumour growth. Nat. Commun. 11, 3653 (2020).

Tadepalle, N., Robers, L., Veronese, M., Zentis, P., Babatz, F., Brodesser, S., Gruszczyk, A., Schauss, A., Höning, S., and Rugarli, E. (2020). Microtubule-dependent and independent roles of spastin in lipid droplet dispersion and biogenesis. Life Sci. Alliance 3, e202000715.

Schütter, M., Giavalisco, P., Brodesser, S., and Graef, M. (2020). Local fatty acid channeling into phospholipid synthesis drives phagophore expansion during autophagy. Cell 180, 135-149.

Herholz, M., Cepeda, E., Baumann, L., Kukat, A., Hermeling, J., Maciej, S., Szczepanowska, K., Pavlenko, V., Frommolt, P., and Trifunovic, A. (2019). KLF-1 orchestrates a xenobiotic detoxification program essential for longevity of mitochondrial mutants. Nat. Commun. 10, 3323.

Murru, S., Hess, S., Barth, E., Almajan, E.R., Schatton, D., Hermans, S., Brodesser, S., Langer, T., Kloppenburg, P., Rugarli, E.I. (2019). Astrocyte-specific deletion of the mitochondrial m-AAA protease reveals glial contribution to neurodegeneration. Glia 67, 1526-1541.

Turpin-Nolan, S.M., Hammerschmidt, P., Chen, W., Jais, A., Timper, K., Awazawa, M., Brodesser, S., Brüning, J.C. (2019). CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes Obesity-Induced Insulin Resistance. Cell Rep. 26, 1-10.e7.

Rashid, T., Nemazanyy, I., Paolini, C., Tatsuta, T., Crespin, P., de Villeneuve, D., Brodesser, S., Benit, P., Rustin, P., Baraibar, M.A., Agbulut, O., Olivier, A., Protasi, F., Langer, T., Chrast, R., de Lonlay, P., de Foucauld, H., Blaauw, B., and Pende, M. (2019). Lipin1 deficiency causes sarcoplasmic reticulum stress and chaperone-responsive myopathy. EMBO J. 38, e99576.

Becker, C., Kukat, A., Szczepanowska, K., Hermans, S., Senft, K., Brandscheid, C.P., Maiti, P., and Trifunovic, A. (2018). CLPP deficiency protects against metabolic syndrome but hinders adaptive thermogenesis. EMBO Rep. 19. DOI: 10.15252/embr.201745126. [Epub ahead of print]

Nolte, H., MacVicar, T.D., Tellkamp, F., Krüger, M. (2018). Instant Clue: A Software Suite for Interactive Data Visualization and Analysis. Sci Rep. 8, 12648.

Wiederstein, J.L., Nolte, H., Günther, S., Piller, T., Baraldo, M., Kostin, S., Bloch, W., Schindler, N., Sandri, M., Blaauw, B., Braun, T., Hölper, S., Krüger, M. (2018). Skeletal Muscle-Specific Methyltransferase METTL21C Trimethylates p97 and Regulates Autophagy-Associated Protein Breakdown. Cell Rep. 23, 1342-1356.