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B08 | Sulfite- and nitrite-dependent regulation of mitochondrial function by sulfite oxidase

Günter Schwarz
Institute for Biochemistry
University of Cologne 

E-mail: gschwarzSpamProtectionuni-koeln.de
Phone: +49 - 221 / 470 6441
For more information and contact please visit the SCHWARZ LAB.


Running time within the CRC 1218: 07/2016 - 06/2024.
 

Abstract

Sulfite oxidase (SOX) is a molybdenum enzyme in the intermembrane space of mitochondria, which catalyses the terminal step in oxidative cysteine catabolism. A loss in SOX function results in severe neurodegeneration, due to cytotoxicity of accumulating sulfite, S-sulfocysteine and H2S leading to mitochondrial dysfunction. Here, we aim to understand the molecular mechanism of sulfite-mediated redox changes and H2S release as well as S-sulfocysteine-induced calcium influx on mitochondrial functions in SOX deficiency. Furthermore, we will investigate the mitochondrial function of SOX in nitrite-dependent NO-synthesis.

Project-related publications

Bender, D., Kaczmarek, A.T., Kuester, S., Burlina, A.B., and Schwarz G. (2020). Oxygen and nitrite reduction by heme-deficient sulphite oxidase in a patient with mild sulphite oxidase deficiency. J. Inherit. Metab. Dis. Jan 17; doi: 10.1002/jimd.12216. [Epub ahead of print]

Mayr, S.J., Röper, J., and Schwarz, G. (2020). Alternative splicing of the bicistronic gene molybdenum cofactor synthesis 1 (MOCS1) uncovers a novel mitochondrial protein maturation mechanism. J. Biol. Chem., Jan 29. pii: jbc.RA119.010720. doi: 10.1074/jbc.RA119.010720. [Epub ahead of print]

Bender, D., Kaczmarek, A.T., Santamaria-Araujo, J.A., Stueve, B., Waltz, S., Bartsch, D., Kurian, L., Cirak, S., and Schwarz, G. (2019). Impaired mitochondrial maturation of sulfite oxidase in a patient with severe sulfite oxidase deficiency. Hum. Mol. Genet. 28, 2885-2899.

Bender, D., Kaczmarek, AT., Niks, D., Hille, R., and Schwarz, G. (2019). Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase. Biochem. J. 476, 1805-1815.

Kohl, J.B., Mellis, A.T., and Schwarz, G. (2019). Homeostatic impact of sulfite and hydrogen sulfide on cysteine catabolism. Br. J. Pharmacol. 176, 554-570.

Kaczmarek, A.T., Strampraad, M.J.F., Hagedoorn, P.L., and Schwarz, G. (2019). Reciprocal regulation of sulfite oxidation and nitrite reduction by mitochondrial sulfite oxidase. Nitric Oxide. 89, 22-31.

Zivanovic, J., Kouroussis, E., Kohl, J.B., ... Schwarz, G., Snyder, S.H., Paul, B.D., Carroll, K.S., Filipovic, M.R. (2019). Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration. Cell Metab. 30, 1152-1170.e13.

Bender, D., and Schwarz, G. (2018). Nitrite-dependent nitric oxide synthesis by molybdenum enzymes. FEBS Lett. 592, 2126-2139.

Kumar, A. Dejanovic, B., Hetsch, F., Semtner, M., Fusca, D., Arjune, S., Santamaria-Araujo, J.A., Winkelmann, A., Ayton, S., Bush, A.I., Kloppenburg, P., Meier, J.C., Schwarz, G., and Belaidi, A.A. (2017). S-sulfocysteine/NMDA receptor-dependent signaling underlies neurodegeneration in molybdenum cofactor deficiency. J. Clin. Invest. 127, 4365-4378.

Schwarz, G. (2016). Molybdenum cofactor and human disease. Curr. Opin. Chem. Biol. 31, 179-187.

Schwahn, B.C., Van Spronsen, F.J., Belaidi, A.A., Bowhay, S., Christodoulou, J., Derks, T.G., Hennermann, J.B., Jameson, E., Konig, K., McGregor, T.L., et al. Schwarz, G.* (2015). Efficacy and safety of cyclic pyranopterin monophosphate substitution in severe molybdenum cofactor deficiency type A: a prospective cohort study. Lancet 386, 1955-1963. * corresponding author