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Z03 | Mitochondrial structure and dynamics in light and electron microscopy

Astrid Schauss
CECAD Research Center/Institute for Genetics
University of Cologne

E-mail: ASchaussSpamProtectionuni-koeln.de
Phone: +49 - 221 / 478 84027
For more information and contact please visit the IMAGING FACILITY.

Ana J. García Sáez
CECAD Research Center/Institute for Genetics
University of Cologne

Phone: +49 221 / 478 84263  
E-mail: ana.garciaSpamProtectionuni-koeln.de
For more information and contact please visit the GARCIA LAB.

Running time within the CRC 1218: 07/2016 - 06/2024.
Ana Garcia joined the project in 2020.



The CECAD Imaging Facility will support the CRC1218 with a Z-project (Z03). Despite our general support in established light and electron microscopy methods, we will set a focus on mitochondrial cristae structures in STED microscopy using SNAP constructs labelled with SNAP-Cell SiR. For electron microscopy, we will set up ApexII and HRP protocols for different mouse tissues, optimize HPF and freeze substitution for different tissues and C. elegans and move from TEM to serial section EM and serial section electron tomography to widen the spectrum to volume imaging. CLEM will be performed combining STED and TEM.

Project-related publications

Ricke, K.M., Paß, T., Kimoloi, S., Fährmann, K., Jüngst, C., Schauss, A., Baris, O.R., Aradjanski, M., Trifunovic, A., Eriksson Faelker, T.M., Bergami, M., Wiesner, R.J. (2020). Mitochondrial Dysfunction Combined with High Calcium Load Leads to Impaired Antioxidant Defense Underlying the Selective Loss of Nigral Dopaminergic Neurons. J Neurosci. 2020 Feb 26;40(9):1975-1986

Anton, V., Buntenbroich, I., Schuster, R., Babatz, F., Simoes, T., Altin, S., Calabrese, G., Riemer, J., Schauss, A.C., Escobar-Henriques, M. (2019). Plasticity in salt-bridge allows fusion-competent ubiquitylation of mitofusins and Cdc48 recognition. Life Sci. Alliance, 2(6). pii: e201900491.

Schatton, D., Pla-Martin, D., Marx, M.C., Hansen, H., Mourier, A., Nemazanyy, I., Pessia, A., Zentis, P., Corona, T., Kondylis, V., Barth, E., Schauss, A.C., Velagapudi, V., and Rugarli, E.I. (2017). CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J. Cell Biol. 216, 675-693. doi: 10.1083/jcb.201607019.

Miller, S.E., Mathiasen, S., Bright, N.A., Pierre, F., Kelly, B.T., Kladt, N., Schauss, A.C., Merrifield, C.J., Stamou, D., Höning, S., and Owen, D.J. (2015). CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. Dev. Cell. 33, 163-175.

Witt, A., Seeger, J.M., Coutelle, O., Zigrino, P., Broxtermann, P., Andree, M., Brinkmann, K., Jüngst, C., Schauss, A.C., Schüll, S., Wohlleber, D., Knolle, P.A., Krönke, M., Mauch, C. and Kashkar, H. (2015) IAP antagonization promotes inflammatory destruction of vascular endothelium. EMBO Rep. 16, 719-27.

Anand, R., Wai, T., Baker, M.J., Kladt, N., Schauss, A.C., Rugarli, E. and Langer, T. (2014). The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. J. Cell Biol. 17, 204, 919-929.